食品合成生物学与生物制造团队

303. Jia DX, Li JH, Liu L, Yang Y, Zhang DX, Du GC, Chen J. High-level expression, purification, and enzymatic characterization of truncated poly (vinyl alcohol) dehydrogenase in methylotrophic yeast Pichia pastoris. Applied Microbiology and Biotechnology [J], 2013, 97: 1113-1120

302. Yang HQ, Liu L, Li JH, Du GC, Chen J. Structure-based engineering of histidine residues in the catalytic domain of α-amylase from Bacillus subtilis for improved protein stability and catalytic efficiency under acidic conditions. Journal of Biotechnology [J], 2013, 164: 59-66

301. Yang HQ, Liu L, Shin HD, Chen RR., Li JH, Du GC, Chen J. Comparative analysis of heterologous expression, biochemical characterization and optimal production of a novel alkaline α-amylase from alkaliphilic Alkalimonas amylolytica in Escherichia coli and Pichia pastoris. Biotechnology Progress [J], 2013, 29: 39-47

300. Liu L, Gazi SH, Shin HD, Li JH, Du GC, Chen J. One-step production of α-ketoglutaric acid from glutamic acid with an engineered L-amino acid deaminase from Proteus mirabilis. Journal of Biotechnology [J], 2013, 164: 97-104

299. Han RZ, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Systems Engineering of Tyrosine 195, Tyrosine 260, and Glutamine265 in Cyclodextrin Glycosyltransferase from Paenibacillus macerans To Enhance Maltodextrin Specificity for 2-O-D-Glucopyranosyl-LAscorbic Acid Synthesis. Applied and Environmental Microbiology [J], 2013, 79: 672-677

298. Jia DX, Liu L, Li JH, Du GC, Chen J. Overproduction of a truncated poly (vinyl alcohol) dehydrogenase in recombinant Pichia pastoris by low-temperature induction strategy and related mechanism analysis. Bioprocess and Biosystems Engineering [J], 2013, 36: 1095-1103

297. Han RZ, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Site-saturation engineering of lysine 47 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance substrate specificity towards maltodextrin for enzymatic synthesis of AA-2G. Applied Microbiology and Biotechnology [J], 2013, 97: 5851-5860

296. Yang HQ, Lu XY, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Fusion of an Oligopeptide to the N Terminus of an Alkaline -Amylase from Alkalimonas amylolytica simultaneously improves the Enzyme’s Catalytic Efficiency, Thermal Stability, and Resistance to Oxidation. Applied and Environmental Microbiology [J], 2013, 79: 3049-3058

295. Yang HQ, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Structure-based systems-level engineering of oxidation-prone methionine residues in catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica for significant improvement of both oxidative stability and catalytic efficiency. PLoS ONE [J], 2013, 8 (3): e57403

294. Liu L, Han RZ, Li JH, Shin HD, Du GC, Chen J. Biosynthesis of 2-O-D-glucopyranosyl-L-ascorbic acid from maltose by an engineered cyclodextrin glycosyltransferase from Paenibacillus macerans. Carbohydrate Research [J], 2013, 382: 101-107

293.Han RZ, Shin HD, Chen RR., Li JH, Du GC, Liu L, Chen J. Carbohydrate binding module fusion of cyclodextrin glycosyltransferase enables the efficient synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid with soluble starch as the glycosyl donor. Applied and Environmental Microbiology [J], 2013, 79: 3234-3240

292. Zhuge X, Li JH, Shin HD, Chen RR., Du GC, Liu L, Chen J. Development of a Propionibacterium-Escherichia coli shuttle vector as a useful tool for metabolic engineering of Propionibacterium jensenii, an efficient producer of propionic acid. Applied and Environmental Microbiology [J], 2013, 79: 4595-4602

291. Liu L, Liu YF, Shin HD, Chen RR., Wang NS, Li JH, Du GC, Chen J. Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology. Applied Microbiology and Biotechnology [J], 2013, 97: 6113-6127

290. Liu L, Xu QY, Shin HD, Chen RR., Li JH, Du GC, Chen J. Improving maltodextrin specificity for enzymatic synthesis of 2-O-D-glucopyranosyl-L-ascorbic acid by site-saturation engineering of subsite -3 in cyclodextrin glycosyltransferase from Paenibacillus maceras. Journal of Biotechnology [J],2013, 166: 198-205

289. Liu L, Liu YF, Ma WL, Zhu YQ, Shin HD, Chen RR, Li JH, Du GC, Chen J. Microbial production of glucosamine and N-acetylglucosamine: Advances and perspectives Applied Microbiology and Biotechnology. 2013, 97: 6149-6158

288. Guan NZ, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen. Systems-level understanding how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: Mechanism and application. Journal of Biotechnology [J], 2013, 167: 56-63

287. Yang HQ, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Integrating terminal truncation and oligopeptide fusion as a novel protein engineering strategy to improve specific activity and catalytic efficiency: Alkaline α-amylase as a case study. Applied and Environmental Microbiology [J], 2013, 79: 6429-6438.

286. Liu YF, Liu L, Shin HD, Chen RR, Li JH, Du GC, Chen J. Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine. Metabolic Engineering. [J], 2013, 19: 107-115.

285. Lu XY, Liu S, Zhang DX, Zhou XM, Wang M, Liu Y, Wu J, Du GC, Chen J. Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides. Applied Microbiology and Biotechnology [J], 2013, 97(21): 9419-9427

284. Chen KK, Liu S, Wang GS, Zhang DX, Du GC, Chen J, Shi ZP. Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide. Journal of Industrial Microbiology & Biotechnology [J], 2013, 40(3-4):782-787

283. Chen KK, Liu S, Zhang DX, Wang M, Shi ZP, Du GC, Chen J. Altered secretary efficiency of Streptomyces hygroscopicus transglutaminase in Escherichia coli by the pro-peptide modification. Process Biochemistry [J], 2013, 48(5-6):782-787

282. Chen KK, Zhang DX, Liu S, Wang NS, Wang M, Du GC, Chen J. Improvement of transglutaminase production by extending differentiation phase of Streptomyces hygroscopicus: mechanism and application. Applied Microbiology and Biotechnology [J], 2013, 97(17): 7711-7719.

281. Lu XY, Zhang J, Liu S, Zhang DX, Xu Z, Wu J, Li J, Du GC, Chen J. Overproduction, purification, and characterization of extracellular lipoxygenase of Pseudomonas aeruginosa in Escherichia coli. Applied Microbiology and Biotechnology [J],2013,97(13): 5793-5800

280. Bi J, Fang F, Lu SY, Du GC, Chen J. New insight into the catalytic properties of bile salt hydrolase. Journal of Molecular Catalysis B:Enzymatic [J],2013,96:46-51

279. Zhang JJ, Kang Z, Ling ZM, Cao WL, Liu L, Wang M, Du GC, Chen J. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements. Bioresource Technology. [J]. 2013, 146:543-8

278. Ling ZM, Liu Y, Teng SL, Kang Z, Zhang JJ, Chen J, Du GC. Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris. Applied and Environmental Microbiology.[J]. 2013, 79(12):3851-5

277. Zhang CZ, Zhang JL, Kang Z, Du GC, Yu XB, Wang TW, Chen J. Enhanced production of L-phenylalanine in Corynebacterium glutamicum due to the introduction of Escherichia coli wild-type. Journal of Industrial Microbiology & Biotechnology.[J]. 2013, 40(6):643-51

276. Wu JJ, Du GC, Zhou JW, Chen J. Metabolic engineering of Escherichia coli for (2S)-pinocembrin production from glucose by a modular metabolic strategy. Metabolic Engineering [J], 2013, 16:48-55

275. Liu JD, Zhu YB, Du GC, Zhou JW, Chen J. Exogenous ergosterol protects Saccharomyces cerevisiae from D-Limonene stress. Journal of Applied Microbiology [J], 2013, 114(2): 482-491

274. Zhou JW, Xu ZH, Chen SW. Simulation and prediction of the thuringiensin abiotic degradation processes in aqueous solution by a radius basis function neural network model. Chemosphpere [J], 2013, 91(4): 442-447

273. Lv YK, Zhao XR, Liu L, Du GC, Zhou JW, Chen J. A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example. Journal of Microbiological Methods [J], 2013, 94: 25-29

272. Liu JD, Zhu YB, Du GC, Zhou JW, Chen J. Response of Saccharomyces cerevisiae to D-limonene-induced oxidative stress. Applied Microbiology and Biotechnology [J], 2013, 97(14): 6467-6475

271. Wang XB, Liu J, Du GC, Zhou JW, Chen J. Efficient production of L-sorbose from D-sorbitol by whole cell immobilization of Gluconobacter oxydans WSH-003. Biochemical Engineering Journal [J], 2013, 77: 171–176

270. Zhao XR, Du GC, Zou HJ, Fu JW, Zhou JW, Chen J. Progress in preventing the accumulation of ethyl carbamate in alcoholic beverages. Trends in Food Science and Technology [J], 2013, 32: 97-107

269. Zhou JW, Zheng QS, Liu J, Huang JM, Du GC, Chen J. Indigenous plasmids of Bacillus megaterium WSH-002 involved in mutualism with Ketogulonicigenium vulgare WSH-001. Plasmid [J], 2013, 70(2):240-246

268. Wu JJ, Liu P, Fan YM, Bao H, Du GC, Zhou JW, Chen J. Multivariate modular metabolic engineering of Escherichia coli to produce resveratrol from L-tyrosine. Journal of Biotechnology [J], 2013, 167: 404-411

267. Zhao XR, Zou HJ, Fu JW, Zhou JW, Chen J, Du GC. Nitrogen regulation involved in the accumulation of urea in Saccharomyces cerevisiae used in rice wine production. Yeast [J], 2013. 30(11): 437-447

266. Liu JD, Zhang WP, Du GC, Chen J, Zhou JW. Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae. Journal of Biotechnology. [J]. 2013, 168(4): 446-451

265. Gu L, Zhang J, Liu BH, Wu CD, Du GC, Chen J. High-level extracellular production of D-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach. Bioprocess and Biosystem Engineering. [J]. 2013 36:1767–1777

264. Liu BH, Zhang J, Li B, Liao XR, Du GC, Chen J. Expression and characterization of extreme alkaline, oxidation-resistant keratinase from Bacillus licheniformis in recombinant Bacillus subtilis WB600 expression system and its application in wool fiber processing. World Journal of Microbiology and Biotechnology. [J]. 2013, 29(5): 825-832

263. Liu BH, Zhang J, Fang Z, Gu , Liao XR, Du GC, Chen J. Enhanced thermostability of keratinase by computational design and empirical mutation. Journal of Industrial Microbiology. [J]. 2013, 40(7): 697-704

262. Fang Z, Zhang J, Liu BH, Du GC, Chen J. Biodegradation of wool waste and keratinase production in scale-up fermenter with different strategies by Stenotrophomonas maltophilia BBE11-1. Bioresource technology. [J]. 2013, 140:286-291

261. Fang Z, Zhang J, Liu BH, Du GC, Chen J. Biochemical characterization of three keratinolytic enzymes from Stenotrophomonas maltophilia BBE11-1 for biodegrading keratin wastes. International Biodeterioration & Biodegradation. [J]. 2013, 82: 166–172

260. Wu CD, Zhang J, Du GC, Chen J. Heterologous expression of Lactobacillus casei RecO improved the multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 during salt stress. Bioresource Technology. [J]. 2013, 143: 238-241

259. Gu L, Zhang J, Liu BH, Wu CD, Du GC, Chen J. High-level extracellular production of D-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach Bioprocess and Biosystem Engineering. (2013) 36:1767–1777

258. Wu CD, Zhang J, Du GC, Chen J. Aspartate protects Lactobacillus casei against acid stress. Applied Microbiology and Biotechnology. [J]. 2013, 97(9): 4083-4093

257. Liu QT, Liu L, Shin HD, Chen RR., Li JH, Du GC, Chen J. Biosynthesis of homoeriodictyol from eriodictyol by flavone 3'-O-methyltransferase from recombinant Yarrowia lioplytica: Heterologous expression, biochemical characterization, and optimal transformation. Journal of Biotechnology. [J]. 2013. 167: 472-478

256. Han RZ, Liu L, Shin HD, Chen RR., Li JH, Du GC, Chen J. Iterative saturation mutagenesis of -6 subsite residues in cyclodextrin glycosyltransferase from Paenibacillus macerans to improve maltodextrin specificity for synthesis 2-O-D-glucopyranosyl-L-ascorbic acid. Applied and Environmental Microbiology. [J]. 2013. 79(24):7562-7568.

255. Liu L, Yang HQ, Shin HD, Li JH, Du GC, Chen J. Recent advances in recombinant protein production by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection. Applied Microbiology and Biotechnology. [J].2013. 97: 9597-9608.

254. Dong ZX, Zhang J, Lee BH, Du GC, Chen J. A bile salt hydrolase gene of Lactobacillus plantarum BBE7 with high cholesterol-removing act. European Food Research and Technology. [J].2012, 35:419-427.

253. Chen KK, Liu S, Ma JL, Zhang DX, Shi ZP, Du GC, Chen J. Deletion combined with saturation mutagenesis of N-terminal residues in transglutaminase from Streptomyces hygroscopicus results in enhanced activity and thermostability. Process Biochemistry. [J].2012, 47:2329-2334.

252. Wu CD, Zhang J, Chen W, Wang M, Du GC, Chen J. A combined phy-siological and proteomic approach to reveal lactic-acid-induced alterations in Lactobacillus casei Zhang and its mutant with enhanced lactic acid tolerance.Applied Microbiology and Biotechnology. [J]. 2012,93:707-722.

251. Zhu YB, Liu JD, Liu J, Du GC, Zhou JW, Chen J. A high throughput method to screen companion bacterium for 2-keto-l-gulonic acid biosynthesis by co-culturing Ketogulonicigenium vulgare Process Biochemistry. [J]. 2012, 47: 1428-1432.

250. Liu L, Liu QF, Li JH, Du GC, Chen J. Characterization of Gelatin and Casein Films Modified by Microbial Transglutaminase and the Application as Coating Agents in Leather Finishing Journal of the American Leather Chemists Association. [J]. 2012.107:13-20.

249. Yang HQ, Liu L, Li JH, Du GC, Chen J. Cloning, heterologous expression,and comparative characterization of a mesophilic α-amylase gene from Bacillus subtilis JN16 in Escherichia coli. Annals of Microbiology. [J].2012, 62:1219-1226.

248. Gao LL, Zhou JW, Liu J, Du GC, Chen J. Draft Genome Sequence of Gluconobacter oxydans WSH-003, a Strain That Is Extremely Tolerant of Saccharides and Alditols. Journal of Bacteriology. [J].2012, 194(16):4455-456.

247. Liu YF, Liu L, Chen JH, Li JH, Du GC, Chen J. Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5. Bioprocess and Biosystems Engineering. [J]. 2012, 35:1209-1218.

246. Zhang J, Wu CD, Du GC, Chen J. Enhanced Acid Tolerance in Lactobacillus casei by Adaptive Evolution and Compared Stress Response during Acid Stress.Biotechnology and Bioprocess Engineering. [J].2012, 17: 283-289.

245. Yu ZZ, Du GC, Zhou JW, Chen J. Enhanced a-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06 by an improved integrated fed-batch strategy. Bioresource Technology. [J].2012, 114: 597-602.

244. Yin XX, Madzak C, Du GC, Zhou JW, Chen J. Enhanced α-ketoglutaric acid production in Yarrowia lipolytica WSH-Z06 by regulation of the pyruvate carboxylation pathway. Applied Microbiology and Biotechnology [J].2012, 96:1527-1537.

243. Zhang JX, Liu L, Li JH, Du GC, Chen J. Enhanced glucosamine production by Aspergillus sp. BCRC 31742 based on the time-variant kinetics analysis of dissolved oxygen level. Bioresource Technology. [J]. 2012.111:507–511

242. Zhou JW, Yin XX, Madzak C, Du GC, Chen J. Enhanced α-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism. Journal of Biotechnology. [J]. 2012.161:257–264

241. Liu H, Li JH, Du GC, Zhou JW, Chen J. Enhanced production of a-cyclodextrin glycosyltransferase in Escherichia coli by systematic codon usage optimization. Journal of Industrial. Microbiology and Biotechnology. [J]. 2012.39:1841–1849

240. Li M, Zhang DX, Du GC, Chen J. Enhancement of PVA-Degrading Enzyme Production by the Application of pH Control Strategy Journal of Industrial Microbiology and Biotechnology. [J]. 2012.22(2): 220–225

239. Yang Y, Zhang DX, Liu S, Jia DX, Du GC, Chen J. Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli. Journal of Industrial Microbiology and Biotechnology. [J]. 2012.39:99–104

238. Ling ZM, Ma TB, Li JH, Du GC, Kang Z, Chen J. Functional expression of trypsin from Streptomyces griseus by Pichia pastoris. Journal of Industrial Microbiology and Biotechnology. [J]. 2012.39:1651–1662

237. Han RZ, Liu L, Li JH, Du GC, Chen J. Functions, applications and production of 2-O-D-glucopyranosyl-L-ascorbic acid. Applied Microbiology and Biotechnology.[J]. 2012.95:313–320

236. Zhang J, Li Y, Chen W, Du GC, Chen J. Glutathione improves the cold resistance of Lactobacillus sanfranciscensis by physiological regulation. Food Microbiology.[J]. 2012.31:285-292

235. Chen X, Liu L, Li JH, Du GC, Chen J. Improved glucosamine and N-acetylglucosamine production by an engineered Escherichia coli via step-wise regulation of dissolved oxygen level. Bioresource Technology. [J]. 2012.110：534–538

234. Wang HL, Li JH, Liu L, Li XM, Jia DX, Du GC, Chen J, Jiangning Song. Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture. Bioresource Technology. [J]. 2012.124：338–346

233. Hu FF, Liu JD, Du GC, Hua ZZ, Zhou JW, Chen J. Key cytomembrane ABC transporters of Saccharomyces cerevisiae fail to improve the tolerance to D-limonene. Biotechnology Letters. [J]. 2012.34:1505–1509

232. Wu CD, Zhang J, Wang M, Du GC, Chen J. Lactobacillus casei combats acid stress by maintaining cell membrane functionality Journal of Industrial. Microbiology and Biotechnology[J]. 2012 39:1031–1039

231. Zhang CY, Liu L, Teng LP, Chen JH, Liu J, Li JH, Du GC, Chen J. Metabolic engineeringof Escherichia coli BL21 forbiosynthesisofheparosan, a bioengineered heparinprecursor. Metabolic Engineering. [J]. 2012.14:521–527

230. Liu L, Zhu YF, Li JH, Wang M, Lee PS, Du GC, Chen J. Microbial production of propionic acid from propionibacteria: Current state, challenges and perspectives. Critical Reviews in Biotechnology. [J] 2012. 32(4): 374–381

229. Chen X, Liu L, Li JH, Liu J, Du GC, Chen J. Optimization of glucose feeding approaches for enhanced glucosamine and N-acetylglucosamine production by an engineered Escherichia coli. Journal of Industrial Microbiology and Biotechnology. [J]. 2012.39:359–365

228. Zhou JW, Liu H, Du GC, Li JH, Chen J. Production of α-Cyclodextrin Glycosyltransferase in Bacillus megaterium MS941 by Systematic Codon Usage Optimization. Journal of Agricultural and Food Chemistry. [J]. 2012.60:10285−10292

227 Kang Z, Zhang JL, Zhou JW, Qi QS, Du GC, Chen J. Recent advances in microbial production of δ-aminolevulinic acid and vitamin B12. Biotechnology Advances.[J]. 2012.30:1533–1542

226. Xu GQ, Liu LM, Chen J. Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae. Microbial Cell Factories. [J]. 2012, 11:24

225. Qiu XF, Wang TW, Zhong XM, Du GC, Chen J. Screening and Characterization of an Aerobic Nitrifying-denitrifying Bacterium from Activated Sludge. Biotechnology and Bioprocess Engineering. [J]. 2012.17: 353-360

224. Zhu YB, Liu J, Du GC, Zhou JW, Chen J. Sporulation and stable spore of Bacillus megaterium enhance Ketogulonigenium vulgare propagation and 2-keto-L-gulonic acid biosynthesis. Bioresource Technology. [J]. 2012. 107: 399-404.

223. Yang HQ, Liu L, Wang MX, Li JH, Wang NS, Du GC, Chen J. Structure-Based Engineering of Methionine Residues in the Catalytic Cores of Alkaline Amylase from Alkalimonas amylolytica for Improved Oxidative Stability. Applied and Environmental Microbiology. [J]. 2012.78(21):7519–7526

222. Yang HQ, Liu L, Li JH, Du GC, Chen J. Structure-Based Replacement of Methionine Residues at the Catalytic Domains with Serine Significantly Improves the Oxidative Stability of Alkaline Amylase from Alkaliphilic Alkalimonas amylolytica. Biotechnology Progress. [J]. 2012.28(5):1271-1277

221. Xu GQ, Zou W, Chen XL, Xu N, Liu LM, Chen J. Fumaric Acid Production in Saccharomyces cerevisiae by In Silico Aided Metabolic Engineering. Plos One. [J]. 2012.7(12): e52086.

220 Duan SB, Yuan GQ, Zhao YL, Li HF, Ni WJ, Sang MN, Liu LM, Shi ZP. Enhanced Cephalosporin C production with a combinational ammonium sulfate and DO-Stat based soybean oil feeding strategy. Biochemical Engineering Journal. [J]. 2012. 61: 1-10

219. Ding J, Cao Y, Enock M, Shi ZP. A hybrid support vector machine and fuzzy reasoning based fault diagnosis and rescue system for stable glutamate fermentation. Chemical Engineering Research and Design. [J]. 2012, 90, 1197-1207

218. Gao MJ, Li Z, Yu RS, Wu JR, Zheng ZY, Shi ZP, Zhan XB. Lin CC. Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shif. Bioprocess and Biosystems Engineering. 2012, 35(7), 1125-1136

217. Li X, Li ZG, Zheng JP, Shi ZP, Li L. Yeast extract promotes phase shift of bio-butanol fermentation by Clostridium acetobutylicum ATCC824 using cassava as substrate. Bioresource Technology. [J]. 2012, 125, 43-51